P-115: The Role of G22A Adenosine Deaminase 1 Gene Polymorphism and The Activities of ADA Isoenzymes in Fertile and Infertile Men

Background Some studies have revealed enzymatic and non-enzymatic roles of adenosine deaminase (ADA) in male reproductive system such as regulating adenylate cyclase activity by decreasing adenosine concentration, helping to interaction between prostasomes and spermatozoa. MaterialsAndMethods frequency distribution of ADA1 G22A alleles and genotypes in 200 fertile and 200 infertile men. The polymerase chain reaction– restriction fragment length polymorphism (PCR-RFLP) technique was used for determining of ADA1 G22A variants. In addition, ADA isoenzymes activities (ADA1 and ADA2) were measured using Giusti and Galanti colorimetric method. Results The frequency of GG genotype was significantly higher and GA genotype was lower in infertile males compared with fertile men (P=0.048 and P=0.045 respectively). However, there was not any noticeable difference in allele distribution between groups (P>0.05). Based on logistic regression analysis, the GA genotype has a protective role and can decrease the risk of male infertility 1.7 times (P=0.046). There were significantly higher activities of ADAT and its isoenzymes in infertile males compared with fertile men (P